The Pontifical Catholic University of Valparaíso (PUCV) was founded in 1928 and is one of the most prestigious institutions of the Chilean higher educational system. With an enrollment of over 13,000 students, the university hosts around 250 international students each semester. Although the PUCV's academic facilities are located in both Valparaíso and Viña del Mar, its main complex is located on the northern edge of Valparaíso across the street from the ISA office. This complex houses administrative offices, classrooms, laboratories, libraries and computer and audio-visual facilities. All international students will have orientation sessions at the university at the start of the semester to finalize course selections. Study abroad students also have access to tutoring, computer, sports and recreation facilities. ISA participants may also participate in the various workshops and organizations that the Pontifical Catholic University offers.

Viña del Mar University (UVM) was founded in 1988 and has an enrollment of 5,000 students among its three campuses. The UVM offers a wide range of facilities and is equipped with all the latest technology. International students attend classes on the Agua Santa Campus, which is conveniently located right in the heart of Viña, only a few minutes from the Valparaíso border.

Universidad Adolfo Ibáñez is an AACSB accredited university which originated from the Business School of Valparaiso in 1953. Since then its faculties have expanded to cover areas of study such as Commercial and Civil Industrial Engineering, Law, Journalism, Psychology, History, Literature, and Government. It has an enrollment of more than 7,000 students among its campuses, and offers undergraduate and graduate degrees. The campus also boasts facilities for playing tennis, soccer, volleyball, rugby, basketball, swimming, weight training and more.